Chapter title |
Taurine Inhibits Kupffer Cells Activation Induced by Lipopolysaccharide in Alcoholic Liver Damaged Rats
|
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Chapter number | 61 |
Book title |
Taurine 10
|
Published in |
Advances in experimental medicine and biology, January 2017
|
DOI | 10.1007/978-94-024-1079-2_61 |
Pubmed ID | |
Book ISBNs |
978-9-40-241077-8, 978-9-40-241079-2
|
Authors |
Gaofeng Wu, Qunhui Yang, Yang Yu, Shumei Lin, Ying Feng, Qiufeng Lv, Jiancheng Yang, Jianmin Hu |
Abstract |
Taurine, a β free amino-acid, takes various biological functions including maintain the normal hepatic structure and function. In this study, the regulation mechanism of taurine on lipopolysaccharide (LPS) induced activation of Kupffer cells (KC) in the liver of rats with alcoholic liver disease (ALD) were explored. Male wistar rats were intragastrically administered with alcohol and pyrazole, and ate high-fat diet in order to establish ALD model. Taurine were administered in drinking water simultaneous with and after ALD model establishment. The preventive trial was lasted for 12 weeks, while the curative trial was lasted for 4 weeks. Finally, blood and liver were collected in order to detect the concentrations of plasma LPS and hepatic tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6). Hepatic total RNA were extracted, gene expressions of LPS binding protein (LBP), leukocyte differentiation antigen 14 (CD14), toll-like receptors (TLR4), nuclear transcription factor (NF-κB) and TNF-α were detected by semi-quantitative RT-PCR. The results showed significant elevated levels of plasma LPS, hepatic TNF-α, IL-1β and IL-6 in ALD rats (P < 0.05), and heightened gene expressions of LBP, CD14, TLR4, NF-κB and TNF-α (P < 0.05); Taurine no matter administered preventively or curatively can reduce the levels of plasma LPS, hepatic TNF-α, IL-1β, IL-6, and down-regulate the gene expressions of LBP, CD14, TLR4, NF-κB and TNF-α. The results demonstrated that taurine can prevent and cure ALD by reducing the production and transformation of LPS as well as inhibiting the opening and the transmission of LPS induced KC activation and the downstream signaling pathway. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 2 | 17% |
Student > Bachelor | 2 | 17% |
Researcher | 2 | 17% |
Student > Master | 2 | 17% |
Professor | 1 | 8% |
Other | 0 | 0% |
Unknown | 3 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 25% |
Medicine and Dentistry | 2 | 17% |
Agricultural and Biological Sciences | 1 | 8% |
Veterinary Science and Veterinary Medicine | 1 | 8% |
Psychology | 1 | 8% |
Other | 1 | 8% |
Unknown | 3 | 25% |