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The Plant Endoplasmic Reticulum

Overview of attention for book
Cover of 'The Plant Endoplasmic Reticulum'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Labeling the ER for Light and Fluorescence Microscopy
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    Chapter 2 3D Electron Microscopy of the ER
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    Chapter 3 Characterization of Proteins Localized to Plant ER-PM Contact Sites
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    Chapter 4 Preparation and Imaging of Specialized ER Using Super-Resolution and TEM Techniques
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    Chapter 5 Quantitation of ER Structure and Function
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    Chapter 6 Long-Term Imaging of Endoplasmic Reticulum Morphology in Embryos During Seed Germination
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    Chapter 7 Dancing with the Stars: Using Image Analysis to Study the Choreography of the Endoplasmic Reticulum and Its Partners and of Movement Within Its Tubules
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    Chapter 8 Preparation of Highly Enriched ER Membranes Using Free-Flow Electrophoresis
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    Chapter 9 ER Microsome Preparation in Arabidopsis thaliana
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    Chapter 10 ER Membrane Lipid Composition and Metabolism: Lipidomic Analysis
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    Chapter 11 2in1 Vectors Improve In Planta BiFC and FRET Analyses
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    Chapter 12 Metabolons on the Plant ER
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    Chapter 13 Using Optical Tweezers Combined with Total Internal Reflection Microscopy to Study Interactions Between the ER and Golgi in Plant Cells
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    Chapter 14 Protein Biosynthesis and Maturation in the ER
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    Chapter 15 ER Membrane Protein Interactions Using the Split-Ubiquitin System (SUS)
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    Chapter 16 Analysis of Protein Glycosylation in the ER
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    Chapter 17 The Unfolded Protein Response
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    Chapter 18 Unfolded Protein Response in Arabidopsis
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    Chapter 19 Fluorescence Imaging of Autophagy-Mediated ER-to-Vacuole Trafficking in Plants
  21. Altmetric Badge
    Chapter 20 Imaging the ER and Endomembrane System in Cereal Endosperm
Attention for Chapter 20: Imaging the ER and Endomembrane System in Cereal Endosperm
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Chapter title
Imaging the ER and Endomembrane System in Cereal Endosperm
Chapter number 20
Book title
The Plant Endoplasmic Reticulum
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7389-7_20
Pubmed ID
29043684
Book ISBNs
978-1-4939-7388-0, 978-1-4939-7389-7
Authors

Verena Ibl, Jenny Peters, Eva Stöger, Elsa Arcalís

Abstract

The cereal endosperm is a complex structure comprising distinct cell types, characterized by specialized organelles for the accumulation of storage proteins. Protein trafficking in these cells is complicated by the presence of several different storage organelles including protein bodies (PBs) derived from the endoplasmic reticulum (ER) and dynamic protein storage vacuoles (PSVs). In addition, trafficking may follow a number of different routes depending on developmental stage, showing that the endomembrane system is capable of massive reorganization. Thus, developmental sequences involve progressive changes of the endomembrane system of endosperm tissue and are characterized by a high structural plasticity and endosomal activity.Given the technical dexterity required to access endosperm tissue and study subcellular structures and (seed storage protein) SSP trafficking in cereal seeds, static images are the state of the art providing a bulk of information concerning the cellular composition of seed tissue. In view of the highly dynamic endomembrane system in cereal endosperm cells, it is reasonable to expect that live cell imaging will help to characterize the spatial and temporal changes of the system. The high resolution achieved with electron microscopy perfectly complements the live cell imaging.We therefore established an imaging platform for TEM as well as for live cell imaging. Here, we describe the preparation of different cereal seed tissues for live cell imaging concomitant with immunolocalization studies and ultrastructure.

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X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 50%
Student > Bachelor 1 25%
Student > Master 1 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 100%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 October 2017.
All research outputs
#14,366,847
of 23,006,268 outputs
Outputs from Methods in molecular biology
#4,222
of 13,160 outputs
Outputs of similar age
#240,401
of 442,254 outputs
Outputs of similar age from Methods in molecular biology
#432
of 1,498 outputs
Altmetric has tracked 23,006,268 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,160 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,254 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,498 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.

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