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The Plant Endoplasmic Reticulum

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Cover of 'The Plant Endoplasmic Reticulum'

Table of Contents

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    Book Overview
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    Chapter 1 Labeling the ER for Light and Fluorescence Microscopy
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    Chapter 2 3D Electron Microscopy of the ER
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    Chapter 3 Characterization of Proteins Localized to Plant ER-PM Contact Sites
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    Chapter 4 Preparation and Imaging of Specialized ER Using Super-Resolution and TEM Techniques
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    Chapter 5 Quantitation of ER Structure and Function
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    Chapter 6 Long-Term Imaging of Endoplasmic Reticulum Morphology in Embryos During Seed Germination
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    Chapter 7 Dancing with the Stars: Using Image Analysis to Study the Choreography of the Endoplasmic Reticulum and Its Partners and of Movement Within Its Tubules
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    Chapter 8 Preparation of Highly Enriched ER Membranes Using Free-Flow Electrophoresis
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    Chapter 9 ER Microsome Preparation in Arabidopsis thaliana
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    Chapter 10 ER Membrane Lipid Composition and Metabolism: Lipidomic Analysis
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    Chapter 11 2in1 Vectors Improve In Planta BiFC and FRET Analyses
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    Chapter 12 Metabolons on the Plant ER
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    Chapter 13 Using Optical Tweezers Combined with Total Internal Reflection Microscopy to Study Interactions Between the ER and Golgi in Plant Cells
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    Chapter 14 Protein Biosynthesis and Maturation in the ER
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    Chapter 15 ER Membrane Protein Interactions Using the Split-Ubiquitin System (SUS)
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    Chapter 16 Analysis of Protein Glycosylation in the ER
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    Chapter 17 The Unfolded Protein Response
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    Chapter 18 Unfolded Protein Response in Arabidopsis
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    Chapter 19 Fluorescence Imaging of Autophagy-Mediated ER-to-Vacuole Trafficking in Plants
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    Chapter 20 Imaging the ER and Endomembrane System in Cereal Endosperm
Attention for Chapter 18: Unfolded Protein Response in Arabidopsis
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Chapter title
Unfolded Protein Response in Arabidopsis
Chapter number 18
Book title
The Plant Endoplasmic Reticulum
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7389-7_18
Pubmed ID
Book ISBNs
978-1-4939-7388-0, 978-1-4939-7389-7
Authors

Cristina Ruberti, Federica Brandizzi

Abstract

The unfolded protein response (UPR) is a highly regulated signaling pathway that is largely conserved across eukaryotes. It is essential for cell homeostasis under environmental and physiological conditions that perturb the protein folding in the endoplasmic reticulum (ER). Arabidopsis is one of the outstanding multicellular model systems in which to investigate the UPR. Here, we described a protocol to induce the UPR in plants, specifically arabidopsis, and to estimate their ability to cope with ER stress through the quantification of physiological parameters.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 18 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 22%
Student > Ph. D. Student 3 17%
Student > Bachelor 3 17%
Lecturer 1 6%
Student > Master 1 6%
Other 1 6%
Unknown 5 28%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 33%
Biochemistry, Genetics and Molecular Biology 5 28%
Psychology 1 6%
Immunology and Microbiology 1 6%
Unknown 5 28%