| Chapter title |
Quantitation of ER Structure and Function
|
|---|---|
| Chapter number | 5 |
| Book title |
The Plant Endoplasmic Reticulum
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7389-7_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7388-0, 978-1-4939-7389-7
|
| Authors |
Mark Fricker, Luke Heaton, Nick Jones, Boguslaw Obara, Stefanie J. Müller, Andreas J. Meyer |
| Abstract |
The plant endoplasmic reticulum forms a network of tubules connected by three-way junctions or sheet-like cisternae. Although the network is three-dimensional, in many plant cells, it is constrained to a thin volume sandwiched between the vacuole and plasma membrane, effectively restricting it to a 2-D planar network. The structure of the network, and the morphology of the tubules and cisternae can be automatically extracted following intensity-independent edge-enhancement and various segmentation techniques to give an initial pixel-based skeleton, which is then converted to a graph representation. Collectively, this approach yields a wealth of quantitative metrics for ER structure and can be used to describe the effects of pharmacological treatments or genetic manipulation. The software is publicly available. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 21% |
| Researcher | 3 | 21% |
| Student > Bachelor | 2 | 14% |
| Professor > Associate Professor | 2 | 14% |
| Student > Master | 1 | 7% |
| Other | 2 | 14% |
| Unknown | 1 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 50% |
| Biochemistry, Genetics and Molecular Biology | 3 | 21% |
| Computer Science | 1 | 7% |
| Design | 1 | 7% |
| Unknown | 2 | 14% |