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Mendeley readers
Chapter title |
Metabolons on the Plant ER
|
---|---|
Chapter number | 12 |
Book title |
The Plant Endoplasmic Reticulum
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7389-7_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7388-0, 978-1-4939-7389-7
|
Authors |
Verena Kriechbaumer, Stanley W. Botchway |
Abstract |
Metabolons are protein complexes that contain all the enzymes necessary for a metabolic pathway but also scaffolding proteins. Such a structure allows efficient channeling of intermediate metabolites from one active site to the next and is highly advantageous for labile or toxic intermediates. Here we describe two methods currently used to identify metabolons via protein-protein interaction methodology: immunoprecipitations using GFP-Trap(®)_A beads to find novel interaction partners and potential metabolon components and FRET-FLIM to test for and quantify protein-protein interactions in planta. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 38% |
Student > Ph. D. Student | 2 | 25% |
Professor | 1 | 13% |
Student > Master | 1 | 13% |
Student > Postgraduate | 1 | 13% |
Other | 0 | 0% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 63% |
Immunology and Microbiology | 1 | 13% |
Engineering | 1 | 13% |
Unknown | 1 | 13% |