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Mendeley readers
Chapter title |
ER Microsome Preparation in Arabidopsis thaliana
|
---|---|
Chapter number | 9 |
Book title |
The Plant Endoplasmic Reticulum
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7389-7_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7388-0, 978-1-4939-7389-7
|
Authors |
Verena Kriechbaumer |
Abstract |
Microsomes are vesicles derived from the endoplasmic reticulum (ER) when cells are broken down in the lab. These microsomes are a valuable tool to study a variety of ER functions such as protein and lipid synthesis in vitro.Here we describe a protocol to isolate ER-derived microsomes Arabidopsis thaliana seedlings and exemplify the use of these purified microsomes in enzyme assays with the auxin precursors tryptophan (Trp) or indole-3-pyruvic acid (IPyA) to quantify auxin synthetic capacity in microsomal and cytosolic fractions. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 2 | 22% |
Student > Bachelor | 1 | 11% |
Student > Postgraduate | 1 | 11% |
Researcher | 1 | 11% |
Unknown | 4 | 44% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 33% |
Biochemistry, Genetics and Molecular Biology | 2 | 22% |
Chemistry | 1 | 11% |
Unknown | 3 | 33% |