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The Plant Endoplasmic Reticulum

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Cover of 'The Plant Endoplasmic Reticulum'

Table of Contents

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    Book Overview
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    Chapter 1 Labeling the ER for Light and Fluorescence Microscopy
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    Chapter 2 3D Electron Microscopy of the ER
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    Chapter 3 Characterization of Proteins Localized to Plant ER-PM Contact Sites
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    Chapter 4 Preparation and Imaging of Specialized ER Using Super-Resolution and TEM Techniques
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    Chapter 5 Quantitation of ER Structure and Function
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    Chapter 6 Long-Term Imaging of Endoplasmic Reticulum Morphology in Embryos During Seed Germination
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    Chapter 7 Dancing with the Stars: Using Image Analysis to Study the Choreography of the Endoplasmic Reticulum and Its Partners and of Movement Within Its Tubules
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    Chapter 8 Preparation of Highly Enriched ER Membranes Using Free-Flow Electrophoresis
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    Chapter 9 ER Microsome Preparation in Arabidopsis thaliana
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    Chapter 10 ER Membrane Lipid Composition and Metabolism: Lipidomic Analysis
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    Chapter 11 2in1 Vectors Improve In Planta BiFC and FRET Analyses
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    Chapter 12 Metabolons on the Plant ER
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    Chapter 13 Using Optical Tweezers Combined with Total Internal Reflection Microscopy to Study Interactions Between the ER and Golgi in Plant Cells
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    Chapter 14 Protein Biosynthesis and Maturation in the ER
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    Chapter 15 ER Membrane Protein Interactions Using the Split-Ubiquitin System (SUS)
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    Chapter 16 Analysis of Protein Glycosylation in the ER
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    Chapter 17 The Unfolded Protein Response
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    Chapter 18 Unfolded Protein Response in Arabidopsis
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    Chapter 19 Fluorescence Imaging of Autophagy-Mediated ER-to-Vacuole Trafficking in Plants
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    Chapter 20 Imaging the ER and Endomembrane System in Cereal Endosperm
Attention for Chapter 17: The Unfolded Protein Response
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Chapter title
The Unfolded Protein Response
Chapter number 17
Book title
The Plant Endoplasmic Reticulum
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7389-7_17
Pubmed ID
Book ISBNs
978-1-4939-7388-0, 978-1-4939-7389-7
Authors

Kazuki Tabara, Yuji Iwata, Nozomu Koizumi

Abstract

Under the unfolded protein response (UPR), transcripts encoding the endoplasmic reticulum (ER) chaperones are increased and those encoding proteins synthesized in the ER are decreased. To reproducibly detect such changes of an expression profile, homogeneous growth of plants is desired. In addition, uniform treatment with drugs inducing the UPR is also necessary. Here we describe our methods of plant culture and drug treatment, and procedure to detect gene expression by quantitative RT-PCR.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 543 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 <1%
India 2 <1%
Australia 1 <1%
Italy 1 <1%
Germany 1 <1%
Mexico 1 <1%
Canada 1 <1%
China 1 <1%
Argentina 1 <1%
Other 0 0%
Unknown 531 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 118 22%
Researcher 82 15%
Student > Bachelor 81 15%
Student > Master 67 12%
Student > Doctoral Student 28 5%
Other 73 13%
Unknown 94 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 181 33%
Agricultural and Biological Sciences 152 28%
Medicine and Dentistry 24 4%
Neuroscience 16 3%
Immunology and Microbiology 16 3%
Other 51 9%
Unknown 103 19%