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Chapter title |
Long-Term Imaging of Endoplasmic Reticulum Morphology in Embryos During Seed Germination
|
---|---|
Chapter number | 6 |
Book title |
The Plant Endoplasmic Reticulum
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7389-7_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7388-0, 978-1-4939-7389-7
|
Authors |
Natasha Dzimitrowicz, Emily Breeze, Lorenzo Frigerio |
Abstract |
Imaging plant embryos at the cellular level over time is technically challenging, since the embryo, once its protective seed coat is removed, must be kept viable and unstressed on a microscope slide for the duration of the experiment. Here we describe a procedure and suitable apparatus for the visualization, over several days, of changes in endoplasmic reticulum (ER) morphology associated with the process of germination in Arabidopsis thaliana seeds. Moreover, we also present a user-friendly image analysis tool which enables subtle perturbations in the ER network to be measured. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 33% |
Student > Ph. D. Student | 2 | 22% |
Student > Master | 2 | 22% |
Unknown | 2 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 56% |
Biochemistry, Genetics and Molecular Biology | 1 | 11% |
Chemistry | 1 | 11% |
Unknown | 2 | 22% |