| Chapter title |
ER Membrane Protein Interactions Using the Split-Ubiquitin System (SUS)
|
|---|---|
| Chapter number | 15 |
| Book title |
The Plant Endoplasmic Reticulum
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7389-7_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7388-0, 978-1-4939-7389-7
|
| Authors |
Lisa Yasmin Asseck, Niklas Wallmeroth, Christopher Grefen |
| Abstract |
Protein-protein interactions (PPIs) play fundamental roles in all cellular processes. Especially membrane proteins facilitate a range of important biological functions in stimuli perception, signaling, and transport. Here we describe a detailed protocol for the yeast mating-based Split-Ubiquitin System (mbSUS) to study PPIs of ER membrane proteins in vivo. In contrast to the prominent Yeast Two-Hybrid, mbSUS enables analysis of full-length membrane proteins in their native cellular context. The system is based on the ubiquitin proteasome pathway leading to the release of an artificial transcription factor followed by activation of reporter genes to visualize PPIs. The mating-based approach is suitable for both small- and large-scale interaction studies. Additionally, we describe protocols to apply the recently established SUS Bridge assay (SUB) which is optimized for the detection of ternary protein interactions. |
X Demographics
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 5 | 42% |
| United Kingdom | 1 | 8% |
| United States | 1 | 8% |
| Austria | 1 | 8% |
| Switzerland | 1 | 8% |
| Unknown | 3 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 6 | 50% |
| Members of the public | 5 | 42% |
| Science communicators (journalists, bloggers, editors) | 1 | 8% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 20% |
| Researcher | 2 | 20% |
| Professor | 1 | 10% |
| Student > Master | 1 | 10% |
| Student > Bachelor | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 3 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 40% |
| Agricultural and Biological Sciences | 3 | 30% |
| Unknown | 3 | 30% |