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The Plant Endoplasmic Reticulum

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Cover of 'The Plant Endoplasmic Reticulum'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Labeling the ER for Light and Fluorescence Microscopy
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    Chapter 2 3D Electron Microscopy of the ER
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    Chapter 3 Characterization of Proteins Localized to Plant ER-PM Contact Sites
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    Chapter 4 Preparation and Imaging of Specialized ER Using Super-Resolution and TEM Techniques
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    Chapter 5 Quantitation of ER Structure and Function
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    Chapter 6 Long-Term Imaging of Endoplasmic Reticulum Morphology in Embryos During Seed Germination
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    Chapter 7 Dancing with the Stars: Using Image Analysis to Study the Choreography of the Endoplasmic Reticulum and Its Partners and of Movement Within Its Tubules
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    Chapter 8 Preparation of Highly Enriched ER Membranes Using Free-Flow Electrophoresis
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    Chapter 9 ER Microsome Preparation in Arabidopsis thaliana
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    Chapter 10 ER Membrane Lipid Composition and Metabolism: Lipidomic Analysis
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    Chapter 11 2in1 Vectors Improve In Planta BiFC and FRET Analyses
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    Chapter 12 Metabolons on the Plant ER
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    Chapter 13 Using Optical Tweezers Combined with Total Internal Reflection Microscopy to Study Interactions Between the ER and Golgi in Plant Cells
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    Chapter 14 Protein Biosynthesis and Maturation in the ER
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    Chapter 15 ER Membrane Protein Interactions Using the Split-Ubiquitin System (SUS)
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    Chapter 16 Analysis of Protein Glycosylation in the ER
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    Chapter 17 The Unfolded Protein Response
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    Chapter 18 Unfolded Protein Response in Arabidopsis
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    Chapter 19 Fluorescence Imaging of Autophagy-Mediated ER-to-Vacuole Trafficking in Plants
  21. Altmetric Badge
    Chapter 20 Imaging the ER and Endomembrane System in Cereal Endosperm
Attention for Chapter 4: Preparation and Imaging of Specialized ER Using Super-Resolution and TEM Techniques
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Chapter title
Preparation and Imaging of Specialized ER Using Super-Resolution and TEM Techniques
Chapter number 4
Book title
The Plant Endoplasmic Reticulum
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7389-7_4
Pubmed ID
Book ISBNs
978-1-4939-7388-0, 978-1-4939-7389-7
Authors

Karen Bell, Karl Oparka, Kirsten Knox

Abstract

The plant endoplasmic reticulum (ER) forms several specialized structures. These include the sieve element reticulum (SER) and the desmotubule formed as the ER passes through plasmodesmata. Imaging both of these structures has been inhibited by the resolution limits of light microscopy and their relatively inaccessible locations, combined with the fragile nature of the ER. Here we describe methods to view desmotubules in live cells under 3D-structured illumination microscopy (3D-SIM) and methods to fix and prepare phloem tissue for both 3D-SIM and transmission electron microscopy (TEM) which preserve the fragile structure and allow the detailed imaging of the SER.

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The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 20%
Student > Postgraduate 1 20%
Other 1 20%
Student > Master 1 20%
Unknown 1 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 40%
Biochemistry, Genetics and Molecular Biology 1 20%
Design 1 20%
Unknown 1 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 November 2017.
All research outputs
#18,574,814
of 23,006,268 outputs
Outputs from Methods in molecular biology
#7,961
of 13,160 outputs
Outputs of similar age
#330,447
of 442,254 outputs
Outputs of similar age from Methods in molecular biology
#950
of 1,498 outputs
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