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Super-Resolution Microscopy

Overview of attention for book
Cover of 'Super-Resolution Microscopy'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Super-Resolution Microscopy Techniques and Their Potential for Applications in Radiation Biophysics
  3. Altmetric Badge
    Chapter 2 Managing the Introduction of Super-Resolution Microscopy into a Core Facility
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    Chapter 3 Live-Cell STED Imaging with the HyPer2 Biosensor
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    Chapter 4 Diffraction-Unlimited Fluorescence Imaging with an EasySTED Retrofitted Confocal Microscope
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    Chapter 5 Two-Photon STED Microscopy for Nanoscale Imaging of Neural Morphology In Vivo
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    Chapter 6 STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live Cells
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    Chapter 7 Four-Channel Super-Resolution Imaging by 3-D Structured Illumination
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    Chapter 8 Correlative SIM-STORM Microscopy
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    Chapter 9 Correlative Super-Resolution Fluorescence Imaging and Atomic Force Microscopy for the Characterization of Biological Samples
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    Chapter 10 Quantitative Single-Molecule Localization Microscopy (qSMLM) of Membrane Proteins Based on Kinetic Analysis of Fluorophore Blinking Cycles
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    Chapter 11 Two-Color Single-Molecule Tracking in Live Cells
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    Chapter 12 Fully Automated Targeted Confocal and Single-Molecule Localization Microscopy
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    Chapter 13 Brain Slice Staining and Preparation for Three-Dimensional Super-Resolution Microscopy
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    Chapter 14 Correlative In-Resin Super-Resolution Fluorescence and Electron Microscopy of Cultured Cells
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    Chapter 15 Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments
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    Chapter 16 Measuring Nanometer Distances Between Fluorescent Labels Step-by-Step
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    Chapter 17 Correlative Single-Molecule Localization Microscopy and Confocal Microscopy
  19. Altmetric Badge
    Chapter 18 Correlative Fluorescence Super-Resolution Localization Microscopy and Platinum Replica EM on Unroofed Cells
  20. Altmetric Badge
    Chapter 19 In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT
  21. Altmetric Badge
    Chapter 20 Super-Resolution High Content Screening and Analysis
Attention for Chapter 2: Managing the Introduction of Super-Resolution Microscopy into a Core Facility
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Chapter title
Managing the Introduction of Super-Resolution Microscopy into a Core Facility
Chapter number 2
Book title
Super-Resolution Microscopy
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7265-4_2
Pubmed ID
Book ISBNs
978-1-4939-7264-7, 978-1-4939-7265-4
Authors

Jeffrey A. Kamykowski, Brian Storrie

Abstract

Super resolution techniques place the resolution of fluorescence microscopy closer to the size of the underlying cell structure or molecular machine being studied. Structured illumination techniques will give users a set of tools that are close to their past experience and relatively simple and quick to learn. The present dyes can be used. Resolution approaching 100 nm XY can be achieved. In contrast, stochastic methods such as PALM/STORM typically require the choice of new dyes and a much greater learning curve to master the technology and calculations. However, a further fivefold resolution improvement is possible. Stimulated depletion techniques such as STED offer a third set of approaches that will again require the use of new dyes. All these approaches require substantial investment in new equipment and in user training. There is no free lunch in the search for better resolution.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 25%
Researcher 2 17%
Student > Bachelor 2 17%
Unspecified 1 8%
Professor 1 8%
Other 1 8%
Unknown 2 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 33%
Engineering 2 17%
Unspecified 1 8%
Neuroscience 1 8%
Agricultural and Biological Sciences 1 8%
Other 0 0%
Unknown 3 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 September 2017.
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#20,447,499
of 23,002,898 outputs
Outputs from Methods in molecular biology
#9,939
of 13,156 outputs
Outputs of similar age
#356,155
of 421,223 outputs
Outputs of similar age from Methods in molecular biology
#842
of 1,074 outputs
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