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Super-Resolution Microscopy

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Cover of 'Super-Resolution Microscopy'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Super-Resolution Microscopy Techniques and Their Potential for Applications in Radiation Biophysics
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    Chapter 2 Managing the Introduction of Super-Resolution Microscopy into a Core Facility
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    Chapter 3 Live-Cell STED Imaging with the HyPer2 Biosensor
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    Chapter 4 Diffraction-Unlimited Fluorescence Imaging with an EasySTED Retrofitted Confocal Microscope
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    Chapter 5 Two-Photon STED Microscopy for Nanoscale Imaging of Neural Morphology In Vivo
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    Chapter 6 STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live Cells
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    Chapter 7 Four-Channel Super-Resolution Imaging by 3-D Structured Illumination
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    Chapter 8 Correlative SIM-STORM Microscopy
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    Chapter 9 Correlative Super-Resolution Fluorescence Imaging and Atomic Force Microscopy for the Characterization of Biological Samples
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    Chapter 10 Quantitative Single-Molecule Localization Microscopy (qSMLM) of Membrane Proteins Based on Kinetic Analysis of Fluorophore Blinking Cycles
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    Chapter 11 Two-Color Single-Molecule Tracking in Live Cells
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    Chapter 12 Fully Automated Targeted Confocal and Single-Molecule Localization Microscopy
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    Chapter 13 Brain Slice Staining and Preparation for Three-Dimensional Super-Resolution Microscopy
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    Chapter 14 Correlative In-Resin Super-Resolution Fluorescence and Electron Microscopy of Cultured Cells
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    Chapter 15 Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments
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    Chapter 16 Measuring Nanometer Distances Between Fluorescent Labels Step-by-Step
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    Chapter 17 Correlative Single-Molecule Localization Microscopy and Confocal Microscopy
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    Chapter 18 Correlative Fluorescence Super-Resolution Localization Microscopy and Platinum Replica EM on Unroofed Cells
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    Chapter 19 In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT
  21. Altmetric Badge
    Chapter 20 Super-Resolution High Content Screening and Analysis
Attention for Chapter 10: Quantitative Single-Molecule Localization Microscopy (qSMLM) of Membrane Proteins Based on Kinetic Analysis of Fluorophore Blinking Cycles
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Chapter title
Quantitative Single-Molecule Localization Microscopy (qSMLM) of Membrane Proteins Based on Kinetic Analysis of Fluorophore Blinking Cycles
Chapter number 10
Book title
Super-Resolution Microscopy
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7265-4_10
Pubmed ID
Book ISBNs
978-1-4939-7264-7, 978-1-4939-7265-4
Authors

Franziska Fricke, Joel Beaudouin, Sebastian Malkusch, Roland Eils, Mike Heilemann, Fricke, Franziska, Beaudouin, Joel, Malkusch, Sebastian, Eils, Roland, Heilemann, Mike

Abstract

Photoswitchable or photoactivatable fluorophores are the key in single-molecule localization microscopy. Next to providing fluorescence images with subdiffraction spatial resolution, additional information is available from observing single fluorophores over time. This includes the characteristic photophysical phenomenon of "blinking" that is exhibited by single fluorescent proteins or fluorophores and follows well-defined kinetic laws. Analyzing the kinetics of "blinking" allows determining the number of fluorophores in a multi-molecular complex. As such, quantitative information at the molecular level can be extracted, representing a tremendously useful extension of single-molecule super-resolution microscopy. This concept is in particular useful to study homo- and heterooligomeric signaling protein complexes in the plasma membrane of an intact cell with molecular resolution. Here, we provide an experimental framework for deciphering the stoichiometry of membrane proteins on the basis of SMLM and photoswitching statistics.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 33%
Unspecified 1 11%
Student > Ph. D. Student 1 11%
Other 1 11%
Student > Master 1 11%
Other 1 11%
Unknown 1 11%
Readers by discipline Count As %
Pharmacology, Toxicology and Pharmaceutical Science 2 22%
Unspecified 1 11%
Agricultural and Biological Sciences 1 11%
Physics and Astronomy 1 11%
Neuroscience 1 11%
Other 2 22%
Unknown 1 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 May 2018.
All research outputs
#18,572,036
of 23,002,898 outputs
Outputs from Methods in molecular biology
#7,959
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Outputs of similar age
#311,407
of 421,223 outputs
Outputs of similar age from Methods in molecular biology
#693
of 1,074 outputs
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