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Super-Resolution Microscopy

Overview of attention for book
Cover of 'Super-Resolution Microscopy'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Super-Resolution Microscopy Techniques and Their Potential for Applications in Radiation Biophysics
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    Chapter 2 Managing the Introduction of Super-Resolution Microscopy into a Core Facility
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    Chapter 3 Live-Cell STED Imaging with the HyPer2 Biosensor
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    Chapter 4 Diffraction-Unlimited Fluorescence Imaging with an EasySTED Retrofitted Confocal Microscope
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    Chapter 5 Two-Photon STED Microscopy for Nanoscale Imaging of Neural Morphology In Vivo
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    Chapter 6 STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live Cells
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    Chapter 7 Four-Channel Super-Resolution Imaging by 3-D Structured Illumination
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    Chapter 8 Correlative SIM-STORM Microscopy
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    Chapter 9 Correlative Super-Resolution Fluorescence Imaging and Atomic Force Microscopy for the Characterization of Biological Samples
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    Chapter 10 Quantitative Single-Molecule Localization Microscopy (qSMLM) of Membrane Proteins Based on Kinetic Analysis of Fluorophore Blinking Cycles
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    Chapter 11 Two-Color Single-Molecule Tracking in Live Cells
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    Chapter 12 Fully Automated Targeted Confocal and Single-Molecule Localization Microscopy
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    Chapter 13 Brain Slice Staining and Preparation for Three-Dimensional Super-Resolution Microscopy
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    Chapter 14 Correlative In-Resin Super-Resolution Fluorescence and Electron Microscopy of Cultured Cells
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    Chapter 15 Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments
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    Chapter 16 Measuring Nanometer Distances Between Fluorescent Labels Step-by-Step
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    Chapter 17 Correlative Single-Molecule Localization Microscopy and Confocal Microscopy
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    Chapter 18 Correlative Fluorescence Super-Resolution Localization Microscopy and Platinum Replica EM on Unroofed Cells
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    Chapter 19 In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT
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    Chapter 20 Super-Resolution High Content Screening and Analysis
Attention for Chapter 7: Four-Channel Super-Resolution Imaging by 3-D Structured Illumination
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Chapter title
Four-Channel Super-Resolution Imaging by 3-D Structured Illumination
Chapter number 7
Book title
Super-Resolution Microscopy
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7265-4_7
Pubmed ID
Book ISBNs
978-1-4939-7264-7, 978-1-4939-7265-4

Ulrike Engel


Multichannel imaging is used as a readout of relative localization of two or more components and is often the first step in investigating functional ensembles in cells. However, the localization volume of diffraction-limited light microscopy (approx. 200 nm by 500 nm) can accommodate hundred of proteins, calling for increased resolution for these types of analyses. Here, we present a protocol for 4-channel imaging using structured illumination microscopy (SIM), which increases resolution by a factor of two. We use adherent, fixed cells to identify the localization of adhesion proteins using immunofluorescence and fluorescent proteins. We discuss how labeling with the necessary brightness is achieved and how data has to be processed for colocalization analysis.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 33%
Student > Doctoral Student 1 33%
Unknown 1 33%
Readers by discipline Count As %
Physics and Astronomy 1 33%
Medicine and Dentistry 1 33%
Unknown 1 33%

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