Chapter title |
Correlative Single-Molecule Localization Microscopy and Confocal Microscopy
|
---|---|
Chapter number | 17 |
Book title |
Super-Resolution Microscopy
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7265-4_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7264-7, 978-1-4939-7265-4
|
Authors |
Christian Soeller, Yufeng Hou, Isuru D. Jayasinghe, David Baddeley, David Crossman, Soeller, Christian, Hou, Yufeng, Jayasinghe, Isuru D., Baddeley, David, Crossman, David |
Abstract |
Single-molecule localization microscopy allows the ability to image fluorescence labeled molecular targets at nanoscale resolution. However, for many biological questions the ability to provide tissue and cellular context in addition to these high resolution data is eminently informative. Here, we describe a procedure to achieve this aim by correlatively imaging human cardiac tissue first at the nanoscale with direct stochastic optical reconstruction microscopy (dSTORM) and then at the diffraction limit with conventional confocal microscopy. |
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