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Super-Resolution Microscopy

Overview of attention for book
Cover of 'Super-Resolution Microscopy'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Super-Resolution Microscopy Techniques and Their Potential for Applications in Radiation Biophysics
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    Chapter 2 Managing the Introduction of Super-Resolution Microscopy into a Core Facility
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    Chapter 3 Live-Cell STED Imaging with the HyPer2 Biosensor
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    Chapter 4 Diffraction-Unlimited Fluorescence Imaging with an EasySTED Retrofitted Confocal Microscope
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    Chapter 5 Two-Photon STED Microscopy for Nanoscale Imaging of Neural Morphology In Vivo
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    Chapter 6 STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live Cells
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    Chapter 7 Four-Channel Super-Resolution Imaging by 3-D Structured Illumination
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    Chapter 8 Correlative SIM-STORM Microscopy
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    Chapter 9 Correlative Super-Resolution Fluorescence Imaging and Atomic Force Microscopy for the Characterization of Biological Samples
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    Chapter 10 Quantitative Single-Molecule Localization Microscopy (qSMLM) of Membrane Proteins Based on Kinetic Analysis of Fluorophore Blinking Cycles
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    Chapter 11 Two-Color Single-Molecule Tracking in Live Cells
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    Chapter 12 Fully Automated Targeted Confocal and Single-Molecule Localization Microscopy
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    Chapter 13 Brain Slice Staining and Preparation for Three-Dimensional Super-Resolution Microscopy
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    Chapter 14 Correlative In-Resin Super-Resolution Fluorescence and Electron Microscopy of Cultured Cells
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    Chapter 15 Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments
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    Chapter 16 Measuring Nanometer Distances Between Fluorescent Labels Step-by-Step
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    Chapter 17 Correlative Single-Molecule Localization Microscopy and Confocal Microscopy
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    Chapter 18 Correlative Fluorescence Super-Resolution Localization Microscopy and Platinum Replica EM on Unroofed Cells
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    Chapter 19 In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT
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    Chapter 20 Super-Resolution High Content Screening and Analysis
Attention for Chapter 6: STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live Cells
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Chapter title
STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live Cells
Chapter number 6
Book title
Super-Resolution Microscopy
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7265-4_6
Pubmed ID
28924659
Book ISBNs
978-1-4939-7264-7, 978-1-4939-7265-4
Authors

Roman S. Erdmann, Derek Toomre, Alanna Schepartz, Erdmann, Roman S., Toomre, Derek, Schepartz, Alanna

Abstract

Long time-lapse super-resolution imaging in live cells requires a labeling strategy that combines a bright, photostable fluorophore with a high-density localization probe. Lipids are ideal high-density localization probes, as they are >100 times more abundant than most membrane-bound proteins and simultaneously demark the boundaries of cellular organelles. Here, we describe Cer-SiR, a two-component, high-density lipid probe that is exceptionally photostable. Cer-SiR is generated in cells via a bioorthogonal reaction of two components: a ceramide lipid tagged with trans-cyclooctene (Cer-TCO) and a reactive, photostable Si-rhodamine dye (SiR-Tz). These components assemble within the Golgi apparatus of live cells to form Cer-SiR. Cer-SiR is benign to cellular function, localizes within the Golgi at a high density, and is sufficiently photostable to enable visualization of Golgi structure and dynamics by 3D confocal or long time-lapse STED microscopy.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 30%
Student > Bachelor 3 15%
Researcher 2 10%
Professor 2 10%
Unspecified 1 5%
Other 2 10%
Unknown 4 20%
Readers by discipline Count As %
Chemistry 6 30%
Biochemistry, Genetics and Molecular Biology 3 15%
Agricultural and Biological Sciences 2 10%
Engineering 2 10%
Physics and Astronomy 1 5%
Other 2 10%
Unknown 4 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 May 2018.
All research outputs
#18,572,036
of 23,002,898 outputs
Outputs from Methods in molecular biology
#7,959
of 13,156 outputs
Outputs of similar age
#311,407
of 421,223 outputs
Outputs of similar age from Methods in molecular biology
#693
of 1,074 outputs
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So far Altmetric has tracked 13,156 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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