Chapter title |
Correlative Fluorescence Super-Resolution Localization Microscopy and Platinum Replica EM on Unroofed Cells
|
---|---|
Chapter number | 18 |
Book title |
Super-Resolution Microscopy
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7265-4_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7264-7, 978-1-4939-7265-4
|
Authors |
Kem A. Sochacki, Justin W. Taraska, Sochacki, Kem A., Taraska, Justin W. |
Abstract |
Platinum replicas of unroofed mammalian cells can be imaged with a transmission electron microscope (TEM) to produce high contrast, high resolution images of the structure of the cytoplasmic side of a plasma membrane. A complementary approach, super-resolution fluorescence localization microscopy, can be used to localize labeled molecules with better than 20 nm precision in cells. Here, we describe a correlative method that couples these two techniques and produces images where localization microscopy data can be used to highlight specific proteins of interest within the structural context of the platinum replica TEM image. This combined method is uniquely suited to investigate the nanometer-scale structural organization of the plasma membrane and its associated organelles and proteins. |
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