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Kinase Signaling Networks

Overview of attention for book
Cover of 'Kinase Signaling Networks'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Optogenetic Control of Ras/Erk Signaling Using the Phy–PIF System
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    Chapter 2 Dissecting Kinase Effector Signaling Using the RapRTAP Methodology
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    Chapter 3 Single-Cell Imaging of ERK Signaling Using Fluorescent Biosensors
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    Chapter 4 Quantification of Cell Signaling Networks Using Kinase Activity Chemosensors
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    Chapter 5 Expression of Recombinant Phosphoproteins for Signal Transduction Studies
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    Chapter 6 Allosteric Modulation of Src Family Kinases with ATP-Competitive Inhibitors
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    Chapter 7 Characterization of Ligand Binding to Pseudokinases Using a Thermal Shift Assay
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    Chapter 8 Proteomic Profiling of Protein Kinase Inhibitor Targets by Mass Spectrometry
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    Chapter 9 Utilizing the Luminex Magnetic Bead-Based Suspension Array for Rapid Multiplexed Phosphoprotein Quantification
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    Chapter 10 High-Content Imaging and RNAi Screens for Investigating Kinase Network Plasticity
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    Chapter 11 Analysis of Drug Resistance Using Kinome-Wide Functional Screens
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    Chapter 12 Identification and Validation of Driver Kinases from Next-Generation Sequencing Data
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    Chapter 13 Label-Free Phosphoproteomic Approach for Kinase Signaling Analysis
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    Chapter 14 Cell-Specific Labeling for Analyzing Bidirectional Signaling by Mass Spectrometry
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    Chapter 15 Characterization of the Phospho-Adhesome by Mass Spectrometry-Based Proteomics
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    Chapter 16 Analysis of Phosphotyrosine Signaling Networks in Lung Cancer Cell Lines
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    Chapter 17 Targeted Analysis of Phosphotyrosine Signaling by Multiple Reaction Monitoring Mass Spectrometry
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    Chapter 18 Phosphoproteomic Analysis of Isolated Mitochondria in Yeast
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    Chapter 19 A Methodology for Comprehensive Analysis of Toll-Like Receptor Signaling in Macrophages
  21. Altmetric Badge
    Chapter 20 Absolute Phosphorylation Stoichiometry Analysis by Motif-Targeting Quantitative Mass Spectrometry
  22. Altmetric Badge
    Chapter 21 Identification of Plant Kinase Substrates Based on Kinase Assay-Linked Phosphoproteomics
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    Chapter 22 Mass Spectrometry Analysis of Spatial Protein Networks by Colocalization Analysis (COLA)
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    Chapter 23 Development of Selected Reaction Monitoring Methods to Systematically Quantify Kinase Abundance and Phosphorylation Stoichiometry in Human Samples
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    Chapter 24 Analysis of Signaling Networks at the Single-Cell Level Using Mass Cytometry
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    Chapter 25 Magnetic Resonance Spectroscopy (MRS)-Based Methods for Examining Cancer Metabolism in Response to Oncogenic Kinase Drug Treatment
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    Chapter 26 Deconstructing the Metabolic Networks of Oncogenic Signaling Using Targeted Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
  28. Altmetric Badge
    Chapter 27 Modeling of Receptor Tyrosine Kinase Signaling: Computational and Experimental Protocols
  29. Altmetric Badge
    Chapter 28 An Interdisciplinary Approach for Designing Kinetic Models of the Ras/MAPK Signaling Pathway
  30. Altmetric Badge
    Chapter 29 Databases and Computational Tools for Evolutionary Analysis of Protein Phosphorylation
  31. Altmetric Badge
    Chapter 30 Informatics Approaches for Predicting, Understanding, and Testing Cancer Drug Combinations
  32. Altmetric Badge
    Chapter 31 Target Inhibition Maps Based on Responses to Kinase Inhibitors
  33. Altmetric Badge
    Chapter 32 Partial Least Squares Regression Models for the Analysis of Kinase Signaling
Attention for Chapter 17: Targeted Analysis of Phosphotyrosine Signaling by Multiple Reaction Monitoring Mass Spectrometry
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Chapter title
Targeted Analysis of Phosphotyrosine Signaling by Multiple Reaction Monitoring Mass Spectrometry
Chapter number 17
Book title
Kinase Signaling Networks
Published in
Methods in molecular biology, July 2017
DOI 10.1007/978-1-4939-7154-1_17
Pubmed ID
28730485
Book ISBNs
978-1-4939-7152-7, 978-1-4939-7154-1
Authors

Leo S. Payne, Paul H. Huang, Payne, Leo S., Huang, Paul H.

Abstract

Phosphoproteomics is an important tool for the unbiased investigation of signaling network activation and has particular application to unraveling aberrant signaling driving cancer progression. However, validating the behavior of specific phosphosites across multiple experimental conditions remains challenging, due to limitations inherent in discovery-based proteomic workflows and the limited availability of high-quality antibodies required for alternative, immunoaffinity-based methods. Targeted phosphoproteomics enables specific phosphosites to be quantified reproducibly across multiple experimental conditions. Importantly, targeted phosphoproteomic assays can be designed rapidly on the basis of data acquired in discovery proteomic experiments and circumvent the requirement of immunoaffinity techniques for reliable antibodies raised to specific, potentially poorly immunogenic phosphopeptides. In the following protocol, we present a method for the relative quantification of phosphosites across multiple experimental conditions and/or technical and biological replicates.

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The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 1 20%
Other 1 20%
Unknown 3 60%
Readers by discipline Count As %
Unspecified 1 20%
Unknown 4 80%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 April 2018.
All research outputs
#15,470,944
of 22,990,068 outputs
Outputs from Methods in molecular biology
#5,380
of 13,150 outputs
Outputs of similar age
#197,805
of 314,579 outputs
Outputs of similar age from Methods in molecular biology
#101
of 270 outputs
Altmetric has tracked 22,990,068 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,150 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
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We're also able to compare this research output to 270 others from the same source and published within six weeks on either side of this one. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.

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