Chapter title |
Dissecting Kinase Effector Signaling Using the RapRTAP Methodology
|
---|---|
Chapter number | 2 |
Book title |
Kinase Signaling Networks
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7154-1_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7152-7, 978-1-4939-7154-1
|
Authors |
Anne-Marie Ray, Jennifer E. Klomp, Kerrie B. Collins, Andrei V. Karginov |
Abstract |
Kinases are involved in a broad spectrum of cell behaviors. A single kinase can interact with different ligands each eliciting a specific cellular response. Dissecting downstream signaling pathways of kinases is a key step to understanding physiological and pathological cell process. However, directing kinase activity to specific substrates remains challenging. Here, we present a new tool to selectively activate a kinase in a specific protein complex in living cells. This technology uses a rapamycin-inducible kinase activation coupled to interaction with FKBP12-binding domain (FRB) tagged protein. Here, we demonstrate application of this method by targeting Src to either p130Cas or FAK and discriminating cell mophodynamic changes downstream each of these signaling complexes. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 1 | 50% |
Unknown | 1 | 50% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 1 | 50% |
Unknown | 1 | 50% |