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Mendeley readers
Chapter title |
Absolute Phosphorylation Stoichiometry Analysis by Motif-Targeting Quantitative Mass Spectrometry
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Chapter number | 20 |
Book title |
Kinase Signaling Networks
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Published in |
Methods in molecular biology, July 2017
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DOI | 10.1007/978-1-4939-7154-1_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7152-7, 978-1-4939-7154-1
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Authors |
Chia-Feng Tsai, Wei-Chi Ku, Yu-Ju Chen, Yasushi Ishihama |
Abstract |
Direct measurement of site-specific phosphorylation stoichiometry can unambiguously distinguish whether the degree of phosphorylation is regulated by upstream kinase/phosphatase activity or by transcriptional regulation to alter protein expression level. Here, we describe a motif-targeting quantitative proteomic approach that integrates dephosphorylation, isotope tag labeling, and enzymatic kinase reaction for large-scale phosphorylation stoichiometry measurement of the human proteome. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 40% |
Professor > Associate Professor | 2 | 40% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 3 | 60% |
Chemistry | 1 | 20% |
Unknown | 1 | 20% |