Chapter title |
Quantification of Cell Signaling Networks Using Kinase Activity Chemosensors
|
---|---|
Chapter number | 4 |
Book title |
Kinase Signaling Networks
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7154-1_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7152-7, 978-1-4939-7154-1
|
Authors |
Beck, Jon R., Harris, Edward N., Stains, Cliff I., Jon R. Beck, Edward N. Harris, Cliff I. Stains |
Abstract |
The ability to directly determine endogenous kinase activity in tissue homogenates provides valuable insights into signaling aberrations that underlie disease phenotypes. When activity data is collected across a panel of kinases, a unique "signaling fingerprint" is generated that allows for discrimination between diseased and normal tissue. Here we describe the use of peptide-based kinase activity sensors to fingerprint the signaling changes associated with disease states. This approach leverages the phosphorylation-sensitive sulfonamido-oxine (Sox) fluorophore to provide a direct readout of kinase enzymatic activity in unfractionated tissue homogenates from animal models or clinical samples. To demonstrate the application of this technology, we focus on a rat model of nonalcoholic fatty liver disease (NAFLD). Sox-based activity probes allow for the rapid and straightforward analysis of changes in kinase enzymatic activity associated with disease states, providing leads for further investigation using traditional biochemical approaches. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 4 | 36% |
Student > Ph. D. Student | 1 | 9% |
Professor > Associate Professor | 1 | 9% |
Other | 1 | 9% |
Unknown | 4 | 36% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 18% |
Biochemistry, Genetics and Molecular Biology | 2 | 18% |
Medicine and Dentistry | 2 | 18% |
Unknown | 5 | 45% |