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Mendeley readers
Chapter title |
Expression of Recombinant Phosphoproteins for Signal Transduction Studies
|
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Chapter number | 5 |
Book title |
Kinase Signaling Networks
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Published in |
Methods in molecular biology, July 2017
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DOI | 10.1007/978-1-4939-7154-1_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7152-7, 978-1-4939-7154-1
|
Authors |
Karl W. Barber, Jesse Rinehart |
Abstract |
Complex signaling cascades are difficult to study in vitro without phosphorylated proteins. Here, we describe a technique for the routine production of recombinant phosphoproteins by directly incorporating phosphoserine as a nonstandard amino acid. This protocol utilizes an optimized phosphoserine orthogonal translation system and an engineered strain of E. coli containing no genomic amber codons. This approach has been used to generate a variety of phosphorylated proteins to understand the role of protein phosphorylation in cell signaling. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 3 | 60% |
Researcher | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 40% |
Agricultural and Biological Sciences | 1 | 20% |
Chemistry | 1 | 20% |
Unknown | 1 | 20% |