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Mendeley readers
Chapter title |
Single-Cell Imaging of ERK Signaling Using Fluorescent Biosensors
|
---|---|
Chapter number | 3 |
Book title |
Kinase Signaling Networks
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Published in |
Methods in molecular biology, July 2017
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DOI | 10.1007/978-1-4939-7154-1_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7152-7, 978-1-4939-7154-1
|
Authors |
Michael Pargett, Taryn E. Gillies, Carolyn K. Teragawa, Breanne Sparta, John G. Albeck |
Abstract |
Single-cell analysis of the mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase (ERK) provides a means to perform highly detailed kinetic studies, assess heterogeneity between cells, and distinguish the subcellular localization of ERK activity. We describe here the methods needed to perform such measurements in a cell type of the investigator's choosing. We discuss the selection of appropriate reporters and provide detailed methods for stably introducing reporters, collecting live-cell data, and automatically extracting quantitative information from individual cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 30 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 9 | 30% |
Researcher | 5 | 17% |
Student > Doctoral Student | 2 | 7% |
Student > Bachelor | 2 | 7% |
Student > Postgraduate | 2 | 7% |
Other | 3 | 10% |
Unknown | 7 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 6 | 20% |
Biochemistry, Genetics and Molecular Biology | 5 | 17% |
Engineering | 4 | 13% |
Medicine and Dentistry | 2 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
Other | 5 | 17% |
Unknown | 7 | 23% |