Chapter title |
Screening Vaccine Formulations in Fresh Human Whole Blood
|
---|---|
Chapter number | 22 |
Book title |
Vaccine Adjuvants
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6445-1_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6443-7, 978-1-4939-6445-1
|
Authors |
Jalil Hakimi, Sepideh Aboutorabian, Frederick To, Salvador F. Ausar, Nausheen Rahman, Roger H. Brookes |
Editors |
Christopher B. Fox |
Abstract |
Monitoring the immunological functionality of vaccine formulations is critical for vaccine development. While the traditional approach using established animal models has been relatively effective, the use of animals is costly and cumbersome, and animal models are not always reflective of a human response. The development of a human-based approach would be a major step forward in understanding how vaccine formulations might behave in humans. Here, we describe a platform methodology using fresh human whole blood (hWB) to monitor adjuvant-modulated, antigen-specific responses to vaccine formulations, which is amenable to analysis by standard immunoassays as well as a variety of other analytical techniques. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 38% |
Student > Ph. D. Student | 1 | 13% |
Student > Bachelor | 1 | 13% |
Other | 1 | 13% |
Unknown | 2 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 25% |
Mathematics | 1 | 13% |
Immunology and Microbiology | 1 | 13% |
Engineering | 1 | 13% |
Unknown | 3 | 38% |