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Chapter title |
Staining and Transfer Techniques for SDS-PAGE Gels to Minimize Oil-in-Water Emulsion Adjuvant Interference
|
---|---|
Chapter number | 20 |
Book title |
Vaccine Adjuvants
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Published in |
Methods in molecular biology, October 2016
|
DOI | 10.1007/978-1-4939-6445-1_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6443-7, 978-1-4939-6445-1
|
Authors |
Alicia M. Schwartz, Michelle Y. Chan, Dawn M. Fedor, Sandra J. Sivananthan, Ryan M. Kramer |
Editors |
Christopher B. Fox |
Abstract |
Adjuvants in modern vaccines boost and shape immune responses and allow for antigen dose-sparing. Analysis of protein antigens in the presence of adjuvants can prove challenging, especially if the adjuvant interferes with visualization of the protein band on an SDS-PAGE gel. In this chapter, a variety of different techniques are presented to mitigate the interference of a nanoemulsion adjuvant, GLA-SE, with different recombinant proteins of varying molecular weight by addressing sample preparation and staining methods. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 17% |
Unknown | 5 | 83% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 33% |
Student > Bachelor | 1 | 17% |
Professor | 1 | 17% |
Unknown | 2 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 50% |
Engineering | 1 | 17% |
Unknown | 2 | 33% |