Chapter title |
Biosynthetic Approaches to Squalene Production: The Case of Yeast
|
---|---|
Chapter number | 7 |
Book title |
Vaccine Adjuvants
|
Published in |
Methods in molecular biology, October 2016
|
DOI | 10.1007/978-1-4939-6445-1_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6443-7, 978-1-4939-6445-1
|
Authors |
Martin Valachovič, Ivan Hapala |
Editors |
Christopher B. Fox |
Abstract |
Squalene is a precursor in the eukaryotic sterol biosynthesis. It is a valuable compound with several human health-related applications. Since the traditional natural resources of squalene are limited, alternatives for the production of squalene on industrial scale have been intensively explored during past years. The yeast Saccharomyces cerevisiae represents an attractive option due to elaborated techniques of genetic and metabolic engineering that can be applied to improve squalene yields. We discuss in this chapter some theoretical aspects of genetic manipulations of the ergosterol biosynthesis pathway aimed at increased squalene production and describe analytical methods for squalene purification and determination of its content in yeast cells. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Japan | 1 | 4% |
China | 1 | 4% |
Unknown | 25 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 19% |
Student > Ph. D. Student | 4 | 15% |
Professor > Associate Professor | 3 | 11% |
Student > Master | 3 | 11% |
Student > Doctoral Student | 1 | 4% |
Other | 1 | 4% |
Unknown | 10 | 37% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 26% |
Biochemistry, Genetics and Molecular Biology | 6 | 22% |
Environmental Science | 2 | 7% |
Chemistry | 1 | 4% |
Engineering | 1 | 4% |
Other | 0 | 0% |
Unknown | 10 | 37% |