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Mendeley readers
Chapter title |
Assessment of Antigen-Specific Cellular Immunogenicity Using Intracellular Cytokine Staining, ELISpot, and Culture Supernatants
|
---|---|
Chapter number | 24 |
Book title |
Vaccine Adjuvants
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Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6445-1_24 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6443-7, 978-1-4939-6445-1
|
Authors |
Elyse A. Beebe, Mark T. Orr |
Editors |
Christopher B. Fox |
Abstract |
Quantification of cytokine production by CD4 and CD8 T cells after in vitro recall stimulation with the immunizing antigen is a powerful approach to characterize the cellular immune responses to immunization. Here we describe three complementary methods for such quantification including flow cytometric analysis of cytokine production by intracellular staining, ELISpot determination of the numbers of cytokine-producing cells, and generation of secreted cytokines and chemokines in culture supernatants for analysis by ELISA and/or cytometric bead arrays. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 11% |
Unknown | 8 | 89% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 3 | 33% |
Researcher | 2 | 22% |
Student > Ph. D. Student | 2 | 22% |
Unknown | 2 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 22% |
Immunology and Microbiology | 2 | 22% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 11% |
Veterinary Science and Veterinary Medicine | 1 | 11% |
Chemistry | 1 | 11% |
Other | 0 | 0% |
Unknown | 2 | 22% |