| Chapter title |
Interactions Between Antigens and Nanoemulsion Adjuvants: Separation and Characterization Techniques
|
|---|---|
| Chapter number | 21 |
| Book title |
Vaccine Adjuvants
|
| Published in |
Methods in molecular biology, October 2016
|
| DOI | 10.1007/978-1-4939-6445-1_21 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6443-7, 978-1-4939-6445-1
|
| Authors |
Michelle Y. Chan, Dawn M. Fedor, Tony Phan, Lucien Barnes V, Ryan M. Kramer |
| Editors |
Christopher B. Fox |
| Abstract |
Determining the association of vaccine components in a formulation is of interest for designing and optimizing well characterized vaccines. Three methods are described to assess interactions between protein antigens and oil-in-water nanoemulsion adjuvants. The methods include (1) ultracentrifugation to measure free versus adjuvant-associated protein, (2) size exclusion chromatography (SEC) to qualitatively assess existing interactions, and (3) Native PAGE as a means to visualize the formulation run in its native state on a polyacrylamide gel. As with many techniques, the methods alone are not definitive, but data from multiple orthogonal assays can provide a more complete picture of protein-adjuvant interactions. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 1 | 17% |
| Other | 1 | 17% |
| Student > Doctoral Student | 1 | 17% |
| Unknown | 3 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 2 | 33% |
| Biochemistry, Genetics and Molecular Biology | 1 | 17% |
| Unknown | 3 | 50% |