Chapter title |
Eliciting Epitope-Specific CD8+ T Cell Response by Immunization with Microbial Protein Antigens Formulated with α-Galactosylceramide: Theory, Practice, and Protocols
|
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Chapter number | 25 |
Book title |
Vaccine Adjuvants
|
Published in |
Methods in molecular biology, October 2016
|
DOI | 10.1007/978-1-4939-6445-1_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6443-7, 978-1-4939-6445-1
|
Authors |
Pavlo Gilchuk, Frances C. Knight, John T. Wilson, Sebastian Joyce |
Editors |
Christopher B. Fox |
Abstract |
CD8+ cytotoxic T lymphocytes confer protection against infectious diseases caused by viruses, bacteria, and parasites. Hence, significant efforts have been invested into devising ways to generate CD8+ T cell-targeted vaccines. Generation of microbe-free protein subunit vaccines requires a thorough knowledge of protective target antigens. Such antigens are proteolytically processed peptides presented by MHC class I molecules. To induce a robust antigen-specific CD8+ T cell response through vaccination, it is essential to formulate the antigen with an effective adjuvant. Here, we describe a versatile method for generating high-frequency antigen-specific CD8+ T cells through immunization of mice using the invariant natural killer T cell agonist α-galactosylceramide as the adjuvant. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor > Associate Professor | 2 | 29% |
Researcher | 2 | 29% |
Student > Doctoral Student | 1 | 14% |
Lecturer | 1 | 14% |
Professor | 1 | 14% |
Other | 0 | 0% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 2 | 29% |
Materials Science | 1 | 14% |
Chemistry | 1 | 14% |
Engineering | 1 | 14% |
Unknown | 2 | 29% |