Chapter title |
Using CRISPR-Cas9 to Study ERK Signaling in Drosophila.
|
---|---|
Chapter number | 26 |
Book title |
ERK Signaling
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6424-6_26 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6422-2, 978-1-4939-6424-6
|
Authors |
Marta Forés, Aikaterini Papagianni, Laura Rodríguez-Muñoz, Gerardo Jiménez |
Editors |
Gerardo Jimenez |
Abstract |
Genome engineering using the clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated nuclease 9 (Cas9) technology is revolutionizing biomedical research. CRISPR-Cas9 enables precise editing of genes in a wide variety of cells and organisms, thereby accelerating molecular studies via targeted mutagenesis, epitope tagging, and other custom genetic modifications. Here, we illustrate the CRISPR-Cas9 methodology by focusing on Capicua (Cic), a nuclear transcriptional repressor directly phosphorylated and inactivated by ERK/MAPK. Specifically, we use CRISPR-Cas9 for targeting an ERK docking site of Drosophila Cic, thus generating ERK-insensitive mutants of this important signaling sensor. |
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Demographic breakdown
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