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Mendeley readers
Chapter title |
Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors.
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Chapter number | 15 |
Book title |
ERK Signaling
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6424-6_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6422-2, 978-1-4939-6424-6
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Authors |
Yannick Blum, Rafael D. Fritz, Hyunryul Ryu, Olivier Pertz |
Editors |
Gerardo Jimenez |
Abstract |
Fluorescence resonance energy transfer (FRET)-based biosensors are powerful tools for measuring spatio-temporal signaling dynamics in single living cells with subcellular resolution. There are quite a number of already existing sensors and this technology is increasingly used to obtain quantitative dynamic datasets. In this chapter, we describe the analysis of endogenous extracellular signal-regulated kinase (ERK) activity in living cells using the EKAR2G (ERK activity reporter second generation) probe. We focus on the generation of stable cell lines expressing the EKAR2G sensor as well as data acquisition and analysis. |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 19 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 26% |
Student > Bachelor | 2 | 11% |
Researcher | 2 | 11% |
Student > Master | 2 | 11% |
Professor | 1 | 5% |
Other | 1 | 5% |
Unknown | 6 | 32% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 7 | 37% |
Neuroscience | 4 | 21% |
Agricultural and Biological Sciences | 1 | 5% |
Engineering | 1 | 5% |
Unknown | 6 | 32% |