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ERK Signaling

Overview of attention for book
ERK Signaling
Springer New York

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 How Genetics Has Helped Piece Together the MAPK Signaling Pathway.
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    Chapter 2 In Vitro Enzyme Kinetics Analysis of EGFR.
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    Chapter 3 High-Throughput Analysis of Mammalian Receptor Tyrosine Kinase Activation in Yeast Cells.
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    Chapter 4 Structural Studies of ERK2 Protein Complexes.
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    Chapter 5 Isolation and Characterization of Intrinsically Active (MEK-Independent) Mutants of Mpk1/Erk.
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    Chapter 6 Assaying Activation and Subcellular Localization of ERK in Cells and Tissues.
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    Chapter 7 Detection and Functional Analysis of SUMO-Modified MEK.
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    Chapter 8 Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag.
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    Chapter 9 High-Throughput In Vitro Identification of Direct MAPK/Erk Substrates.
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    Chapter 10 Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGE.
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    Chapter 11 Analysis of Ras/ERK Compartmentalization by Subcellular Fractionation.
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    Chapter 12 Cell-Based Assays to Study ERK Pathway/Caveolin1 Interactions.
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    Chapter 13 The Nuclear Translocation of ERK.
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    Chapter 14 Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA).
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    Chapter 15 Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors.
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    Chapter 16 Quantifying Tensile Force and ERK Phosphorylation on Actin Stress Fibers.
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    Chapter 17 Co-culture Activation of MAP Kinase in Drosophila S2 Cells.
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    Chapter 18 ERK Signaling
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    Chapter 19 3D Organotypic Culture Model to Study Components of ERK Signaling.
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    Chapter 20 Genetic Validation of Cell Proliferation via Ras-Independent Activation of the Raf/Mek/Erk Pathway.
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    Chapter 21 Genome-Wide Analysis of RAS/ERK Signaling Targets.
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    Chapter 22 Probing Chromatin Modifications in Response to ERK Signaling.
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    Chapter 23 Analyzing pERK Activation During Planarian Regeneration.
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    Chapter 24 Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development.
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    Chapter 25 Reconstructing ERK Signaling in the Drosophila Embryo from Fixed Images.
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    Chapter 26 Using CRISPR-Cas9 to Study ERK Signaling in Drosophila.
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    Chapter 27 Analyzing ERK Signal Dynamics During Zebrafish Somitogenesis.
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    Chapter 28 Modeling RASopathies with Genetically Modified Mouse Models.
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    Chapter 29 Dissecting Cell-Fate Determination Through Integrated Mathematical Modeling of the ERK/MAPK Signaling Pathway.
Attention for Chapter 16: Quantifying Tensile Force and ERK Phosphorylation on Actin Stress Fibers.
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Chapter title
Quantifying Tensile Force and ERK Phosphorylation on Actin Stress Fibers.
Chapter number 16
Book title
ERK Signaling
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6424-6_16
Pubmed ID
27924570
Book ISBNs
978-1-4939-6422-2, 978-1-4939-6424-6
Authors

Hiroaki Hirata, Mukund Gupta, Sri Ram Krishna Vedula, Chwee Teck Lim, Benoit Ladoux, Masahiro Sokabe

Editors

Gerardo Jimenez

Abstract

ERK associates with the actin cytoskeleton, and the actin-associated pool of ERK can be activated (phosphorylated in the activation loop) to induce specific cell responses. Increasing evidence has shown that mechanical conditions of cells significantly affect ERK activation. In particular, tension developed in the actin cytoskeleton has been implicated as a critical mechanism driving ERK signaling. However, a quantitative study of the relationship between actin tension and ERK phosphorylation is missing. In this chapter, we describe our novel methods to quantify tensile force and ERK phosphorylation on individual actin stress fibers. These methods have enabled us to show that ERK is activated on stress fibers in a tensile force-dependent manner.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Professor 5 45%
Student > Ph. D. Student 1 9%
Researcher 1 9%
Unknown 4 36%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 18%
Agricultural and Biological Sciences 1 9%
Physics and Astronomy 1 9%
Engineering 1 9%
Unknown 6 55%

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