Chapter title |
Quantitative Analysis of Periodontal Pathogens Using Real-Time Polymerase Chain Reaction (PCR)
|
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Chapter number | 11 |
Book title |
Oral Biology
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Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6685-1_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6683-7, 978-1-4939-6685-1
|
Authors |
Mª José Marin, Elena Figuero, David Herrera, Mariano Sanz |
Editors |
Gregory J. Seymour, Mary P. Cullinan, Nicholas C.K. Heng |
Abstract |
The quantitative polymerase chain reaction (qPCR) is a variant of PCR aimed to detect and quantify a targeted DNA molecule through the addition of probes labeled with fluorescent molecules that emit fluorescence within each amplification cycle, what results in fluorescence values proportional to the amount of accumulated PCR product. This chapter presents the detailed procedures for quantification of different periodontal pathogens (Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Campylobacter rectus, and Fusobacterium spp.) using qPCR. It also includes the description of the most frequent problems encountered and how to solve them. In addition, a detailed protocol for multiplex qPCR to detect and quantify P. gingivalis and A. actinomycetemcomitans is included. |
X Demographics
Geographical breakdown
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor | 2 | 13% |
Student > Master | 2 | 13% |
Student > Doctoral Student | 1 | 7% |
Student > Ph. D. Student | 1 | 7% |
Student > Bachelor | 1 | 7% |
Other | 2 | 13% |
Unknown | 6 | 40% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 6 | 40% |
Agricultural and Biological Sciences | 1 | 7% |
Biochemistry, Genetics and Molecular Biology | 1 | 7% |
Unknown | 7 | 47% |