Chapter title |
RNA Sequencing Analysis of Salivary Extracellular RNA
|
---|---|
Chapter number | 2 |
Book title |
Oral Biology
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6685-1_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6683-7, 978-1-4939-6685-1
|
Authors |
Blanca Majem, Feng Li, Jie Sun, David T. W. Wong, Majem, Blanca, Li, Feng, Sun, Jie, Wong, David T W, David T.W. Wong |
Editors |
Gregory J. Seymour, Mary P. Cullinan, Nicholas C.K. Heng |
Abstract |
Salivary biomarkers for disease detection, diagnostic and prognostic assessments have become increasingly well established in recent years. In this chapter we explain the current leading technology that has been used to characterize salivary non-coding RNAs (ncRNAs) from the extracellular RNA (exRNA) fraction: HiSeq from Illumina® platform for RNA sequencing. Therefore, the chapter is divided into two main sections regarding the type of the library constructed (small and long ncRNA libraries), from saliva collection, RNA extraction and quantification to cDNA library generation and corresponding QCs. Using these invaluable technical tools, one can identify thousands of ncRNA species in saliva. These methods indicate that salivary exRNA provides an efficient medium for biomarker discovery of oral and systemic diseases. |
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Immunology and Microbiology | 1 | 4% |
Other | 3 | 12% |
Unknown | 3 | 12% |