| Chapter title |
A Method to Isolate, Purify, and Characterize Human Periodontal Ligament Stem Cells
|
|---|---|
| Chapter number | 24 |
| Book title |
Oral Biology
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6685-1_24 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6683-7, 978-1-4939-6685-1
|
| Authors |
Krzysztof Mrozik, Stan Gronthos, Songtao Shi, P. Mark Bartold, Mrozik, Krzysztof, Gronthos, Stan, Shi, Songtao, Bartold, P. Mark |
| Editors |
Gregory J. Seymour, Mary P. Cullinan, Nicholas C.K. Heng |
| Abstract |
Human periodontal ligament stem cells (PDLSCs) are a unique population of mesenchymal stem cells (MSCs) that demonstrate the capacity to generate cementum- and periodontal ligament-like structures in vivo. As such, PDLSCs represent a promising cell-based therapy in reconstructive dentistry for the treatment of periodontal disease. The present chapter describes two methods for isolating PDLSCs from human PDL tissue including traditional plastic adherence, and immunomagnetic selection based on the expression of MSC-associated surface markers STRO-1 antigen, CD146 (MUC-18), CD29 (Integrin β-1), CD44, and CD106 (VCAM-1). Although no single antibody demonstrates specificity for MSCs, isolation based on expression of individual markers results in homogenous preparations of PDLSCs. Methods to further characterize the immunophenotype and multipotent capacity of PDLSCs to differentiate into adipocytes, osteoblast-, and cementoblast-like cells in vitro, and cementum- and periodontal ligament-like tissues in vivo, are also described. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 29 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 17% |
| Student > Ph. D. Student | 4 | 14% |
| Student > Bachelor | 3 | 10% |
| Student > Doctoral Student | 2 | 7% |
| Student > Postgraduate | 2 | 7% |
| Other | 5 | 17% |
| Unknown | 8 | 28% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 12 | 41% |
| Biochemistry, Genetics and Molecular Biology | 4 | 14% |
| Materials Science | 1 | 3% |
| Psychology | 1 | 3% |
| Unknown | 11 | 38% |