Chapter title |
Proteomic Analysis of Dental Tissue Microsamples
|
---|---|
Chapter number | 28 |
Book title |
Oral Biology
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6685-1_28 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6683-7, 978-1-4939-6685-1
|
Authors |
Jonathan E. Mangum, Jew C. Kon, Michael J. Hubbard, Mangum, Jonathan E., Kon, Jew C., Hubbard, Michael J. |
Editors |
Gregory J. Seymour, Mary P. Cullinan, Nicholas C.K. Heng |
Abstract |
Improved understanding of dental enamel development will benefit not only dentistry but also biomedicine more generally. Rat and mouse models of enamel development are relatively well characterized and experimentally powerful. However, the diminutive size of murine teeth makes them difficult to study using standard proteomics approaches. Here, we describe gel-based proteomic methods that enable parallel quantification, identification, and functional characterization of proteins from developing rat and mouse teeth. These refined methods are applicable to other scarce samples including human enamel defects. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor | 1 | 33% |
Researcher | 1 | 33% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 1 | 33% |
Medicine and Dentistry | 1 | 33% |
Unknown | 1 | 33% |