| Chapter title |
Generating Multiple Base-Resolution DNA Methylomes Using Reduced Representation Bisulfite Sequencing
|
|---|---|
| Chapter number | 16 |
| Book title |
Oral Biology
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6685-1_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6683-7, 978-1-4939-6685-1
|
| Authors |
Aniruddha Chatterjee, Euan J. Rodger, Peter A. Stockwell, Gwenn Le Mée, Ian M. Morison |
| Editors |
Gregory J. Seymour, Mary P. Cullinan, Nicholas C.K. Heng |
| Abstract |
Reduced representation bisulfite sequencing (RRBS) is an effective technique for profiling genome-wide DNA methylation patterns in eukaryotes. RRBS couples size selection, bisulfite conversion, and second-generation sequencing to enrich for CpG-dense regions of the genome. The progressive improvement of second-generation sequencing technologies and reduction in cost provided an opportunity to examine the DNA methylation patterns of multiple genomes. Here, we describe a protocol for sequencing multiple RRBS libraries in a single sequencing reaction to generate base-resolution methylomes. Furthermore, we provide a brief guideline for base-calling and data analysis of multiplexed RRBS libraries. These strategies will be useful to perform large-scale, genome-wide DNA methylation analysis. |
Mendeley readers
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|---|---|---|
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Demographic breakdown
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|---|---|---|
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| Researcher | 2 | 13% |
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| Other | 1 | 7% |
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| Unknown | 5 | 33% |