Chapter title |
Growing Adipose-Derived Stem Cells Under Serum-Free Conditions
|
---|---|
Chapter number | 26 |
Book title |
Oral Biology
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6685-1_26 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6683-7, 978-1-4939-6685-1
|
Authors |
Diogo Godoy Zanicotti, Dawn E. Coates |
Editors |
Gregory J. Seymour, Mary P. Cullinan, Nicholas C.K. Heng |
Abstract |
Growing adipose-derived stem cells (ADSC) in serum-free conditions is important as it represents a way of expanding multipotent cells in a clinical grade medium. Most cultured ADSC are expanded and tested in serum-containing media, which can pose significant health risks if these cells were used in clinical applications. Moreover, cells grown in serum-free conditions behave significantly different than those cultured in serum-containing media. Here, we present a technique to culture adipose-derived stem cells in serum-free conditions. The methods described in this chapter were optimized for ovine ADSC. The appropriate optimization should be done for other cell lines. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 2 | 33% |
Researcher | 2 | 33% |
Student > Doctoral Student | 1 | 17% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Chemical Engineering | 2 | 33% |
Medicine and Dentistry | 2 | 33% |
Biochemistry, Genetics and Molecular Biology | 1 | 17% |
Unknown | 1 | 17% |