| Chapter title |
CRISPR/Cas9-Mediated Genome Editing of Mouse Small Intestinal Organoids.
|
|---|---|
| Chapter number | 1 |
| Book title |
Gastrointestinal Physiology and Diseases
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3603-8_1 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3601-4, 978-1-4939-3603-8
|
| Authors |
Gerald Schwank, Hans Clevers, Schwank, Gerald, Clevers, Hans |
| Editors |
Andrei I. Ivanov |
| Abstract |
The CRISPR/Cas9 system is an RNA-guided genome-editing tool that has been recently developed based on the bacterial CRISPR-Cas immune defense system. Due to its versatility and simplicity, it rapidly became the method of choice for genome editing in various biological systems, including mammalian cells. Here we describe a protocol for CRISPR/Cas9-mediated genome editing in murine small intestinal organoids, a culture system in which somatic stem cells are maintained by self-renewal, while giving rise to all major cell types of the intestinal epithelium. This protocol allows the study of gene function in intestinal epithelial homeostasis and pathophysiology and can be extended to epithelial organoids derived from other internal mouse and human organs. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | <1% |
| Germany | 1 | <1% |
| Unknown | 120 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 28 | 23% |
| Researcher | 23 | 19% |
| Student > Bachelor | 16 | 13% |
| Student > Master | 12 | 10% |
| Student > Doctoral Student | 7 | 6% |
| Other | 16 | 13% |
| Unknown | 20 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 43 | 35% |
| Agricultural and Biological Sciences | 28 | 23% |
| Immunology and Microbiology | 14 | 11% |
| Medicine and Dentistry | 12 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 2% |
| Other | 3 | 2% |
| Unknown | 19 | 16% |