Chapter title |
Gastrointestinal Physiology and Diseases
|
---|---|
Chapter number | 20 |
Book title |
Gastrointestinal Physiology and Diseases
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3603-8_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3601-4, 978-1-4939-3603-8
|
Authors |
Berek, Claudia, Beller, Alexander, Chu, Van Trung, Claudia Berek, Alexander Beller, Van Trung Chu |
Editors |
Andrei I. Ivanov |
Abstract |
Only recently has it become apparent that eosinophils play a crucial role in mucosal immune homeostasis. Although eosinophils are the main cellular component of the lamina propria of the gastrointestinal tract, they have often been overlooked because they express numerous markers, which are normally used to characterize macrophages and/or dendritic cells. To study their function in mucosal immunity, it is important to isolate them with high purity and viability. Here, we describe a protocol to purify eosinophils from the lamina propria of the murine small intestine. The method involves preparation of the small intestine, removal of epithelial cells and digestion of the lamina propria to release eosinophils. A protocol to sort eosinophils is included. |
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