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Gastrointestinal Physiology and Diseases

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Cover of 'Gastrointestinal Physiology and Diseases'

Table of Contents

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    Book Overview
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    Chapter 1 CRISPR/Cas9-Mediated Genome Editing of Mouse Small Intestinal Organoids.
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    Chapter 2 Lentivirus-Based Stable Gene Delivery into Intestinal Organoids.
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    Chapter 3 Co-culture of Gastric Organoids and Immortalized Stomach Mesenchymal Cells.
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    Chapter 4 An Air-Liquid Interface Culture System for 3D Organoid Culture of Diverse Primary Gastrointestinal Tissues.
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    Chapter 5 Organotypical Tissue Cultures from Fetal and Neonatal Murine Colon.
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    Chapter 6 Ussing Chamber Technique to Measure Intestinal Epithelial Permeability.
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    Chapter 7 HPLC-Based Metabolomic Analysis of Normal and Inflamed Gut.
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    Chapter 8 Gastrointestinal Physiology and Diseases
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    Chapter 9 Gastrointestinal Physiology and Diseases
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    Chapter 10 Gastrointestinal Physiology and Diseases
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    Chapter 11 Gastrointestinal Physiology and Diseases
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    Chapter 12 Label-Free Imaging of Eosinophilic Esophagitis Mouse Models Using Optical Coherence Tomography.
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    Chapter 13 Near-Infrared Fluorescence Endoscopy to Detect Dysplastic Lesions in the Mouse Colon.
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    Chapter 14 Visualization of Signaling Molecules During Neutrophil Recruitment in Transgenic Mice Expressing FRET Biosensors.
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    Chapter 15 In Vivo Myeloperoxidase Imaging and Flow Cytometry Analysis of Intestinal Myeloid Cells.
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    Chapter 16 Gastrointestinal Physiology and Diseases
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    Chapter 17 Gastrointestinal Physiology and Diseases
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    Chapter 18 Purification and Adoptive Transfer of Group 3 Gut Innate Lymphoid Cells.
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    Chapter 19 Immunotherapy with iTreg and nTreg Cells in a Murine Model of Inflammatory Bowel Disease.
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    Chapter 20 Gastrointestinal Physiology and Diseases
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    Chapter 21 Investigation of Host and Pathogen Contributions to Infectious Colitis Using the Citrobacter rodentium Mouse Model of Infection.
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    Chapter 22 Gastrointestinal Physiology and Diseases
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    Chapter 23 Oxazolone-Induced Colitis as a Model of Th2 Immune Responses in the Intestinal Mucosa.
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    Chapter 24 The Mongolian Gerbil: A Robust Model of Helicobacter pylori-Induced Gastric Inflammation and Cancer.
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    Chapter 25 A Rapid Screenable Assay for Compounds That Protect Against Intestinal Injury in Zebrafish Larva.
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    Chapter 26 AOM/DSS Model of Colitis-Associated Cancer.
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    Chapter 27 Characterization of Colorectal Cancer Development in Apc (min/+) Mice.
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    Chapter 28 Modeling Murine Gastric Metaplasia Through Tamoxifen-Induced Acute Parietal Cell Loss.
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    Chapter 29 The Hamster Buccal Pouch Model of Oral Carcinogenesis.
Attention for Chapter 5: Organotypical Tissue Cultures from Fetal and Neonatal Murine Colon.
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Chapter title
Organotypical Tissue Cultures from Fetal and Neonatal Murine Colon.
Chapter number 5
Book title
Gastrointestinal Physiology and Diseases
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3603-8_5
Pubmed ID
Book ISBNs
978-1-4939-3601-4, 978-1-4939-3603-8
Authors

Peter H. Neckel, Lothar Just Ph.D., Lothar Just

Editors

Andrei I. Ivanov

Abstract

The complex functions of the gastrointestinal tract rely on the coordinated interplay of several cell and tissue types involving epithelium, connective tissue, smooth muscles as well as cells of the immune and nervous system. It is therefore obvious, that these functions can hardly be investigated sufficiently using cell lines or two-dimensional cell cultures.Here, we describe an easy to produce three-dimensional organotypical explants culture from fetal and neonatal murine colon. This model is suitable for in vitro testing of intestinal function or the evaluation of developmental or pathological processes.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 29 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 7%
Student > Ph. D. Student 1 3%
Unknown 26 90%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 3%
Nursing and Health Professions 1 3%
Medicine and Dentistry 1 3%
Unknown 26 90%