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Chapter title |
Organotypical Tissue Cultures from Fetal and Neonatal Murine Colon.
|
---|---|
Chapter number | 5 |
Book title |
Gastrointestinal Physiology and Diseases
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3603-8_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3601-4, 978-1-4939-3603-8
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Authors |
Peter H. Neckel, Lothar Just Ph.D., Lothar Just |
Editors |
Andrei I. Ivanov |
Abstract |
The complex functions of the gastrointestinal tract rely on the coordinated interplay of several cell and tissue types involving epithelium, connective tissue, smooth muscles as well as cells of the immune and nervous system. It is therefore obvious, that these functions can hardly be investigated sufficiently using cell lines or two-dimensional cell cultures.Here, we describe an easy to produce three-dimensional organotypical explants culture from fetal and neonatal murine colon. This model is suitable for in vitro testing of intestinal function or the evaluation of developmental or pathological processes. |
Mendeley readers
The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 29 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 2 | 7% |
Student > Ph. D. Student | 1 | 3% |
Unknown | 26 | 90% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 3% |
Nursing and Health Professions | 1 | 3% |
Medicine and Dentistry | 1 | 3% |
Unknown | 26 | 90% |