Chapter title |
An Air-Liquid Interface Culture System for 3D Organoid Culture of Diverse Primary Gastrointestinal Tissues.
|
---|---|
Chapter number | 4 |
Book title |
Gastrointestinal Physiology and Diseases
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3603-8_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3601-4, 978-1-4939-3603-8
|
Authors |
Xingnan Li, Akifumi Ootani, Calvin Kuo |
Editors |
Andrei I. Ivanov |
Abstract |
Conventional in vitro analysis of gastrointestinal epithelium usually relies on two-dimensional (2D) culture of epithelial cell lines as monolayer on impermeable surfaces. However, the lack of context of differentiation and tissue architecture in 2D culture can hinder the faithful recapitulation of the phenotypic and morphological characteristics of native epithelium. Here, we describe a robust long-term three-dimensional (3D) culture methodology for gastrointestinal culture, which incorporates both epithelial and mesenchymal/stromal components into a collagen-based air-liquid interface 3D culture system. This system allows vigorously expansion of primary gastrointestinal epithelium for over 60 days as organoids with both proliferation and multilineage differentiation, indicating successful long-term intestinal culture within a microenvironment accurately recapitulating the stem cell niche. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 106 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 24 | 23% |
Student > Ph. D. Student | 19 | 18% |
Student > Doctoral Student | 9 | 8% |
Student > Bachelor | 7 | 7% |
Student > Master | 6 | 6% |
Other | 13 | 12% |
Unknown | 28 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 28 | 26% |
Agricultural and Biological Sciences | 16 | 15% |
Immunology and Microbiology | 9 | 8% |
Medicine and Dentistry | 9 | 8% |
Unspecified | 2 | 2% |
Other | 9 | 8% |
Unknown | 33 | 31% |