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Chapter title |
In Vivo Myeloperoxidase Imaging and Flow Cytometry Analysis of Intestinal Myeloid Cells.
|
---|---|
Chapter number | 15 |
Book title |
Gastrointestinal Physiology and Diseases
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3603-8_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3601-4, 978-1-4939-3603-8
|
Authors |
Jan Hülsdünker, Robert Zeiser M.D., Robert Zeiser |
Editors |
Andrei I. Ivanov |
Abstract |
Myeloperoxidase (MPO) imaging is a non-invasive method to detect cells that produce the enzyme MPO that is most abundant in neutrophils, macrophages, and inflammatory monocytes. While lacking specificity for any of these three cell types, MPO imaging can provide guidance for further flow cytometry-based analysis of tissues where these cell types reside. Isolation of leukocytes from the intestinal tract is an error-prone procedure. Here, we describe a protocol for intestinal leukocyte isolation that works reliable in our hands and allows for flow cytometry-based analysis, in particular of neutrophils. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 2 | 33% |
Student > Ph. D. Student | 1 | 17% |
Researcher | 1 | 17% |
Student > Master | 1 | 17% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 4 | 67% |
Neuroscience | 1 | 17% |
Unknown | 1 | 17% |