Chapter title |
Co-culture of Gastric Organoids and Immortalized Stomach Mesenchymal Cells.
|
---|---|
Chapter number | 3 |
Book title |
Gastrointestinal Physiology and Diseases
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3603-8_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3601-4, 978-1-4939-3603-8
|
Authors |
Nina Bertaux-Skeirik, Jomaris Centeno, Rui Feng, Michael A. Schumacher, Ramesh A. Shivdasani, Yana Zavros Ph.D., Yana Zavros |
Editors |
Andrei I. Ivanov |
Abstract |
Three-dimensional primary epithelial-derived gastric organoids have recently been established as an important tool to study gastric development, physiology, and disease. Specifically, mouse-derived fundic gastric organoids (mFGOs) co-cultured with Immortalized Stomach Mesenchymal Cells (ISMCs) reflect expression patterns of mature fundic cell types seen in vivo, thus allowing for long-term in vitro studies of gastric epithelial cell physiology, regeneration, and bacterial-host interactions. Here, we describe the development and culture of mFGOs, co-cultured with ISMCs. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 29 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Doctoral Student | 6 | 21% |
Researcher | 5 | 17% |
Student > Ph. D. Student | 4 | 14% |
Professor | 2 | 7% |
Other | 2 | 7% |
Other | 4 | 14% |
Unknown | 6 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 7 | 24% |
Medicine and Dentistry | 7 | 24% |
Agricultural and Biological Sciences | 6 | 21% |
Immunology and Microbiology | 1 | 3% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
Other | 2 | 7% |
Unknown | 5 | 17% |