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Gastrointestinal Physiology and Diseases

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Cover of 'Gastrointestinal Physiology and Diseases'

Table of Contents

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    Book Overview
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    Chapter 1 CRISPR/Cas9-Mediated Genome Editing of Mouse Small Intestinal Organoids.
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    Chapter 2 Lentivirus-Based Stable Gene Delivery into Intestinal Organoids.
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    Chapter 3 Co-culture of Gastric Organoids and Immortalized Stomach Mesenchymal Cells.
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    Chapter 4 An Air-Liquid Interface Culture System for 3D Organoid Culture of Diverse Primary Gastrointestinal Tissues.
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    Chapter 5 Organotypical Tissue Cultures from Fetal and Neonatal Murine Colon.
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    Chapter 6 Ussing Chamber Technique to Measure Intestinal Epithelial Permeability.
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    Chapter 7 HPLC-Based Metabolomic Analysis of Normal and Inflamed Gut.
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    Chapter 8 Gastrointestinal Physiology and Diseases
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    Chapter 9 Gastrointestinal Physiology and Diseases
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    Chapter 10 Gastrointestinal Physiology and Diseases
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    Chapter 11 Gastrointestinal Physiology and Diseases
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    Chapter 12 Label-Free Imaging of Eosinophilic Esophagitis Mouse Models Using Optical Coherence Tomography.
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    Chapter 13 Near-Infrared Fluorescence Endoscopy to Detect Dysplastic Lesions in the Mouse Colon.
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    Chapter 14 Visualization of Signaling Molecules During Neutrophil Recruitment in Transgenic Mice Expressing FRET Biosensors.
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    Chapter 15 In Vivo Myeloperoxidase Imaging and Flow Cytometry Analysis of Intestinal Myeloid Cells.
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    Chapter 16 Gastrointestinal Physiology and Diseases
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    Chapter 17 Gastrointestinal Physiology and Diseases
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    Chapter 18 Purification and Adoptive Transfer of Group 3 Gut Innate Lymphoid Cells.
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    Chapter 19 Immunotherapy with iTreg and nTreg Cells in a Murine Model of Inflammatory Bowel Disease.
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    Chapter 20 Gastrointestinal Physiology and Diseases
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    Chapter 21 Investigation of Host and Pathogen Contributions to Infectious Colitis Using the Citrobacter rodentium Mouse Model of Infection.
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    Chapter 22 Gastrointestinal Physiology and Diseases
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    Chapter 23 Oxazolone-Induced Colitis as a Model of Th2 Immune Responses in the Intestinal Mucosa.
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    Chapter 24 The Mongolian Gerbil: A Robust Model of Helicobacter pylori-Induced Gastric Inflammation and Cancer.
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    Chapter 25 A Rapid Screenable Assay for Compounds That Protect Against Intestinal Injury in Zebrafish Larva.
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    Chapter 26 AOM/DSS Model of Colitis-Associated Cancer.
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    Chapter 27 Characterization of Colorectal Cancer Development in Apc (min/+) Mice.
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    Chapter 28 Modeling Murine Gastric Metaplasia Through Tamoxifen-Induced Acute Parietal Cell Loss.
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    Chapter 29 The Hamster Buccal Pouch Model of Oral Carcinogenesis.
Attention for Chapter 3: Co-culture of Gastric Organoids and Immortalized Stomach Mesenchymal Cells.
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Chapter title
Co-culture of Gastric Organoids and Immortalized Stomach Mesenchymal Cells.
Chapter number 3
Book title
Gastrointestinal Physiology and Diseases
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3603-8_3
Pubmed ID
Book ISBNs
978-1-4939-3601-4, 978-1-4939-3603-8
Authors

Nina Bertaux-Skeirik, Jomaris Centeno, Rui Feng, Michael A. Schumacher, Ramesh A. Shivdasani, Yana Zavros Ph.D., Yana Zavros

Editors

Andrei I. Ivanov

Abstract

Three-dimensional primary epithelial-derived gastric organoids have recently been established as an important tool to study gastric development, physiology, and disease. Specifically, mouse-derived fundic gastric organoids (mFGOs) co-cultured with Immortalized Stomach Mesenchymal Cells (ISMCs) reflect expression patterns of mature fundic cell types seen in vivo, thus allowing for long-term in vitro studies of gastric epithelial cell physiology, regeneration, and bacterial-host interactions. Here, we describe the development and culture of mFGOs, co-cultured with ISMCs.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 29 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 6 21%
Researcher 5 17%
Student > Ph. D. Student 4 14%
Professor 2 7%
Other 2 7%
Other 4 14%
Unknown 6 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 24%
Medicine and Dentistry 7 24%
Agricultural and Biological Sciences 6 21%
Immunology and Microbiology 1 3%
Pharmacology, Toxicology and Pharmaceutical Science 1 3%
Other 2 7%
Unknown 5 17%