Chapter title |
Characterization of Colorectal Cancer Development in Apc (min/+) Mice.
|
---|---|
Chapter number | 27 |
Book title |
Gastrointestinal Physiology and Diseases
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3603-8_27 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3601-4, 978-1-4939-3603-8
|
Authors |
ILKe Nalbantoglu, Valerie Blanc, Nicholas O. Davidson |
Editors |
Andrei I. Ivanov |
Abstract |
The Apc (min/+) mouse provides an excellent experimental model for studying genetic, environmental, and therapeutic aspects of intestinal neoplasia in humans. In this chapter, we will describe techniques for studying colon cancer development in Apc (min/+) mice on C57BL/6J (B6) background, focusing on the roles of environmental modifiers, including Dextran Sulfate Sodium (DSS), high fat diet, and bile acid supplementation in the context of experimental colorectal cancer. This chapter also includes protocols describing extraction and purification of DSS-contaminated RNA, as well as sampling, harvesting, and tissue processing. The common pathologic lesions encountered in these animals are described in detail. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 36 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 13 | 36% |
Researcher | 6 | 17% |
Student > Bachelor | 4 | 11% |
Student > Postgraduate | 2 | 6% |
Student > Master | 2 | 6% |
Other | 4 | 11% |
Unknown | 5 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 10 | 28% |
Agricultural and Biological Sciences | 8 | 22% |
Immunology and Microbiology | 3 | 8% |
Medicine and Dentistry | 3 | 8% |
Sports and Recreations | 1 | 3% |
Other | 2 | 6% |
Unknown | 9 | 25% |