Chapter title |
Bioluminescence
|
---|---|
Chapter number | 4 |
Book title |
Bioluminescence
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3813-1_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3811-7, 978-1-4939-3813-1
|
Authors |
Kim, Sung-Bae, Fujii, Rika, Sung-Bae Kim, Rika Fujii |
Editors |
Sung Bae Kim |
Abstract |
The present protocol introduces fabrication of artificial luciferases (ALuc(®)) by extracting the consensus amino acids from the alignment of copepod luciferase sequences. The made ALucs have unique sequential identities that are phylogenetically distinctive from those of any existing copepod luciferase. Some ALucs exhibited heat stability, and strong and greatly prolonged optical intensities. The made ALucs are applicable to various bioassays as an optical readout, including live cell imaging, single-chain probes, and bioluminescent tags of antibodies. The present protocol guides on how to fabricate a unique artificial luciferase with designed optical properties and functionalities. |
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