| Chapter title |
Circular Permutation Probes for Illuminating Phosphorylation of Estrogen Receptor.
|
|---|---|
| Chapter number | 13 |
| Book title |
Bioluminescence
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3813-1_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3811-7, 978-1-4939-3813-1
|
| Authors |
Sung-Bae Kim, Hiroaki Tao |
| Editors |
Sung Bae Kim |
| Abstract |
The present protocol demonstrates a new strategy for imaging ligand-triggered protein phosphorylation using circularly permutated luciferases (cpLuc): (1) a luciferase is first fragmented into two segments for creating new N- and C-terminal ends in the hydrophilic region, (2) the original N- and C-terminal ends are circularly permutated and linked via a GS linker, whereas the new ends made by fragmentation are correspondingly linked with two proteins of interest. When the new ends of the cpLuc are linked with the ligand-binding domain of estrogen receptor (ER LBD) and Src homology two domain of Src (SH2), the estrogen can trigger phosphorylation of the ER LBD and consequent intramolecular ER LBD-SH2 binding. This interaction triggers an approximation of the adjacent fragments of split-cpLuc recovering the enzyme activity. This probe design greatly improves signal-to-noise (S/N) ratios upon tracing weak protein-protein interactions (PPIs) in mammalian cells. |
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