You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
| Chapter title |
Fabrication of Molecular Strain Probes for Illuminating Protein-Protein Interactions.
|
|---|---|
| Chapter number | 14 |
| Book title |
Bioluminescence
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3813-1_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3811-7, 978-1-4939-3813-1
|
| Authors |
Sung-Bae Kim, Rika Fujii |
| Editors |
Sung Bae Kim |
| Abstract |
A unique bioluminescent imaging probe is introduced for illuminating molecular tension appended by protein-protein interactions (PPIs) of interest. A full-length luciferase is sandwiched between two proteins of interest via minimal flexible linkers. The ligand-activated PPIs append intramolecular tension to the sandwiched luciferase, boosting or dropping the enzymatic activity in a quantitative manner. This method guides construction of a new lineage of bioassays for ligand-activated PPIs. |