| Chapter title |
Bioluminescent Ligand-Receptor Binding Assays for Protein or Peptide Hormones.
|
|---|---|
| Chapter number | 6 |
| Book title |
Bioluminescence
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3813-1_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3811-7, 978-1-4939-3813-1
|
| Authors |
Ya-Li Liu, Zhan-Yun Guo |
| Editors |
Sung Bae Kim |
| Abstract |
Bioluminescence has been widely used in biomedical research due to its high sensitivity, low background, and broad linear range. In recent studies, we applied bioluminescence to ligand-receptor binding assays for some protein or peptide hormones based on a newly developed small monomeric Nanoluciferase (NanoLuc) reporter that has the so far brightest bioluminescence. The conventional ligand-receptor binding assays rely on radioligands that have drawbacks, such as radioactive hazards and short shelf lives. In contrast, the novel bioluminescent binding assays use the NanoLuc-based protein or peptide tracers that are safe, stable, and ultrasensitive. Thus, the novel bioluminescent ligand-receptor binding assay would be applied to more and more protein or peptide hormones for ligand-receptor interaction studies in future. In the present article, we provided detailed protocols for setting up the novel bioluminescent ligand-receptor binding assays using two representative protein hormones as examples. |
Mendeley readers
Geographical breakdown
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|---|---|---|
| United States | 1 | 11% |
| Unknown | 8 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 3 | 33% |
| Student > Bachelor | 1 | 11% |
| Other | 1 | 11% |
| Student > Master | 1 | 11% |
| Professor > Associate Professor | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 2 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Environmental Science | 1 | 11% |
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| Agricultural and Biological Sciences | 1 | 11% |
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| Other | 1 | 11% |
| Unknown | 2 | 22% |