| Chapter title |
Bioluminescence
|
|---|---|
| Chapter number | 3 |
| Book title |
Bioluminescence
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3813-1_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3811-7, 978-1-4939-3813-1
|
| Authors |
Takenaka, Yasuhiro, Ikeo, Kazuho, Shigeri, Yasushi, Yasuhiro Takenaka, Kazuho Ikeo, Yasushi Shigeri |
| Editors |
Sung Bae Kim |
| Abstract |
Secreted luciferases isolated from copepod crustaceans are frequently used for nondisruptive reporter-gene assays, such as the continuous, automated and/or high-throughput monitoring of gene expression in living cells. All known copepod luciferases share highly conserved amino acid residues in two similar, repeated domains in the sequence. The similarity in the domains are ideal nature for designing PCR primers to amplify cDNA fragments of unidentified copepod luciferases from bioluminescent copepod crustaceans. Here, we introduce how to establish a cDNA encoding novel copepod luciferases from a copepod specimen by PCR with degenerated primers. |
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| Unknown | 2 | 67% |
Demographic breakdown
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| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
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|---|---|---|
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| Unknown | 9 | 90% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
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| Unknown | 2 | 20% |
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|---|---|---|
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| Unknown | 2 | 20% |