Chapter title |
Photon Counting System for High-Sensitivity Detection of Bioluminescence at Optical Fiber End.
|
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Chapter number | 25 |
Book title |
Bioluminescence
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3813-1_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3811-7, 978-1-4939-3813-1
|
Authors |
Masataka Iinuma, Yutaka Kadoya, Akio Kuroda |
Editors |
Sung Bae Kim |
Abstract |
The technique of photon counting is widely used for various fields and also applicable to a high-sensitivity detection of luminescence. Thanks to recent development of single photon detectors with avalanche photodiodes (APDs), the photon counting system with an optical fiber has become powerful for a detection of bioluminescence at an optical fiber end, because it allows us to fully use the merits of compactness, simple operation, highly quantum efficiency of the APD detectors. This optical fiber-based system also has a possibility of improving the sensitivity to a local detection of Adenosine triphosphate (ATP) by high-sensitivity detection of the bioluminescence. In this chapter, we are introducing a basic concept of the optical fiber-based system and explaining how to construct and use this system. |
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